Objective:To study expression of MMP-9,Survivin,COX-2,Ki67 in gastric carcinoma and their correlation with angiogenesis and biologic behaviors of tumor.Methods:In 58 gastric carcinoma tissues and 58 normal gastric tissues,the expression of MMP-9、Survivin、COX-2、Ki67 were examined by using strep tavidin-biotin peroxidase(SP) method.Results:The positive expression of rates of MMP-9,Survivin and COX-2 in gastric carcinoma were significantly higher than those in normal gastric mucosa(P<0.01 ).MMP-9,Survivin,COX-2 expression were negative correlated to age,gender,tumor position and diameter of tumors(P>0.05).There was a relationship between MMP-9 gene expression with degrees of differentiation(P<0.05),MMP-9,Survivin,COX-2 expression in gastric carcinoma had relation to other prognostic parameters,such as tumor depth,lymph node metastases and TNM stages(P<0.05).The Ki-67 LI in MMP-9,Survivin and COX-2 positive cases were higher than in MMP-9,Survivin and COX-2 negative cases(P<0.01).There were relationship in MMP-9,Survivin and COX-2(P<0.05).Conclusions:The present study demonstrates that overexpression of MMP-9,Survivin and COX-2 is related to tumor invasion and lymph node metastasis in gastric carcinoma.It indicated MMP-9,Survivin、COX-2、Ki67 maybe ideal tumor markers for early diagnosis and prognosis.