Objective:To investigate the AGT gene polymorphism distribution and the relationship between the AGT gene polymorphism and essential hypertension(EH)in Shenyang Hans population using the new microchip-based method,then to develop a microchip-bases electrophoretic method for rapid detection of genotype.Methods:One hundred and twenty-three patients with EH and one hundred and three controls recruited from Shenyang Hans population respectively were examined.The gene polymorphisms of AGT[G(-6)A]was determined by polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP)with microchip electrophoresis and agarose gel electrophoresis.Results:(1)Microchip electrophoresis could separate PCR products of AGT gene within 250s.(2)The A allele frequency was very high in both groups(control:0.7038,hypertensive:0.7073),it was almost identical in the different groups(χ2=0.024,P>0.05),there was also no significant difference in frequencies of the allele of G(-6)A between Shenyang and Tibetan population(P>0.05).Conclusions:(1)In comparison with gel electrophoresis the new microchip-based method had the features such as high seperation efficiency,smaller sample volume requirement,high-throughput application as well as the sensitivity of detection.(2)Shenyang Hans population had higher frequency of A allele in AGT gene(-6)site.G/A polymorphism exists at locus-6 of human AGT gene,which was not linked to hypertension in Shenyang Han people.