目的 探讨应用荧光定量聚合酶链反应(FQ\|PCR)法检测男性乙肝患者血清和精液中HBV DNA含量的临床意义。方法 选取男性乙肝患者70例，分别采集患者的精液和静脉血，并用FQ\|PCR法检测精液及血清中HBV DNA含量。结果 精液标本中HBV DNA阳性16例（占2286％），HBV DNA含量为255×103～618×105IU／ml、平均为508×104IU／ml；血清标本中HBV DNA阳性62例（占8857％），HBV DNA含量为121×103～752×107IU／ml、平均为940 ×105／ml。精液中HBV DNA含量与血清中HBV DNA含量呈正相关(r=0743)，且血清HBV DNA含量高于精液含量10倍左右；结论 FQ\|PCR法定量检测精液中HBV DNA含量具有较强的特异性和灵敏度，为临床了解乙肝患者精液中HBV状态提供了帮助，具有重要的临床意义。
 

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