中国冶金工业医学杂志

人肝癌细胞株SMMC-7721凋亡的影响研究*

单位：1沈阳医学院环境卫生学教研室辽宁沈阳 110034 2沈阳医学院卫生毒理学教研室

关键词：关键词：氯化镉氯化锌 N\|乙酰半胱氨酸人肝癌细胞株SMMC-7721 凋亡

分类号：中图分类号：R 7357文献标志码：A文章编号：1005-54

出版年,卷(期):页码：2012,29(5):513-515

摘要：

摘要：目的研究氯化锌（ZnCl2）和N\|乙酰半胱氨酸（N\|acetyl cysteine,NAC）对氯化镉(CdCl2)致人肝癌细胞SMMC\|7721增殖及凋亡的影响。方法体外培养的人肝癌细胞SMMC\|7721分别以0、20、40、80、160和320 μmol/L的CdCl2处理3、6、12、24、48 h，ZnCl2预处理和NAC预处理组在CdCl2处理前分别以100 μmol/L的ZnCl2和5 mmol/L的NAC预处理24 h,应用MTT法检测细胞相对存活率，流式细胞仪Annexin V\|PI双标法检测细胞凋亡百分率。结果随着镉处理浓度的增高和时间的延长，SMMC\|7721细胞存活率逐渐降低；SMMC\|7721细胞凋亡率随着CdCl2浓度的增加而逐渐增加，40 μmol/L及以上镉处理组与对照组相比，凋亡率显著增加，P＜0.01；与相同剂量的单纯Cd处理组相比，Zn预处理组细胞凋亡率差异无统计学意义，P＞0.05，与40、80 μmol/L镉处理组相比，相同剂量的NAC预处理组细胞凋亡百分率显著下降，P＜0.05，其余镉处理剂量的NAC预处理组细胞凋亡百分率差异无统计学意义，P＞0.05。结论一定剂量的ZnCl2预处理不能显著降低镉诱导的SMMC\|7721细胞凋亡，而NAC预处理可在一定程度上显著降低镉诱导的肝癌细胞凋亡百分率。

Abstract:ObjectiveTo study the effect of Zinc chloride and N\|acetyl cysteine (NAC) on proliferation and apoptosis of human hepatocellular carcinoma cell SMMC\|7721 induced by cadmium chlorideMethodsSMMC\|7721 cell were incubated with 0,20,40,80,160,and 320μmol/L CdCl2 for 3 h,6 h,12 h 24 h and 48 hThe cells in groups of zinc and NAC pretreatment were treated with 100 μmol/L of ZnCl2 and 5mmol/L NAC for 24 h before incubated with CdCl2The relative viability was observed by MTTThe rate of apoptosis was measured by Flowcytometry Staining of cells with phosphatidylserine(PS)\|annexin\|VResultsCells viability were time and dose dependent decreased with the dose increase of cadmiumCompared with 0μmol/L cadmium,the apoptosis rate increased obviously in the incubating cell with 40、80、160、320μmol/L cadmium(P＜001)Zinc pretreatment did not show the inhibition effects on the apoptosis rate of SMMC\|7721(P＞005)Compared with CdCl2 group,the apoptosis rate of NAC pretreatment group were significantly decreased only when the CdCl2 dose were 40 and 80μmol/L(P＜005)ConclusionZinc chloride could not reduce the apoptosis rate of SMMC\|7721 while NAC might reduce the apoptosis rate of SMMC\|7721 induced by cadmium chloride at certain concentration

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参考文献：

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