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电子期刊 >> 正文 |
| 蛋白激酶受体2在糖尿病大鼠肾组织中的表达及意义 |
| 作者：蔡志杰1 高政南2 程丽静 3 |
| 单位：1内蒙古医科大学第三附属医院老年病科 内蒙古 包头 0140102大连市中心医院内分泌科大连医科大学第二附属医院肾内科 |
| 关键词：蛋白激酶受体2 糖尿病肾病 肾间质纤维化 |
| 分类号： |
| 出版年,卷(期):页码：2014,31(3):249-251 |
| 摘要： |
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摘要：目的通过观察蛋白激酶受体2（PAR\|2）在糖尿病(DM)大鼠肾组织中的表达情况，探讨其在糖尿病肾病肾小管间质纤维化中的可能作用。方法将60只雄性Sprague\|Dawley大鼠随机分为对照组（C组）、糖尿病组（DM组）和用药组(T组)，DM组和T组大鼠腹腔注射链脲佐菌素55 mg/kg制备DM模型，分别于实验的第2、4、8、12、20周处死大鼠后取肾组织制片，在电镜下观察肾组织形态改变；采用免疫组化检测四型胶原（ColⅣ）蛋白和PAR\|2在肾组织中表达情况，并采用病理图像分析软件进行半定量分析。结果C组大鼠肾组织ColⅣ表达均微弱；DM组大鼠肾组织ColⅣ表达逐渐增强，与C组比较差异有统计学意义；T组大鼠肾组织ColⅣ表达增强后又逐渐减弱，与DM组比较差异有统计学意义。C组大鼠肾组织无PAR\|2表达或仅微量表达于血管内皮；DM组大鼠在实验的第2周开始出现PAR\|2表达，并随实验进程PAR\|2表达逐渐增强，与 C组比较差异有统计学意义；T组大鼠肾组织PAR\|2的表达与DM组比较差异无统计学意义。结论PAR\|2早期表达于糖尿病肾病大鼠的近曲小管上皮细胞，肾组织中PAR\|2表达的变化趋势提示PAR\|2可能参与了糖尿病肾病的肾间质纤维化过程。 |
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Abstract:ObjectiveTo observe the expression of protease activated receptor\|2 in kidney of diabetic nephropathy rats and to investigate its contribution to the progression of diabetic in tubulointerstitial fibrosis of diabetic nephropathy ratsMethodsStreptozotocin\|induced diabetic rats were sacrificed at 2w,4w,8d,12w and 20w respectivelyThe renal pathological changes were examined with protein expression of collagen Ⅳ (Col Ⅳ)The protein expression of PAR\|2 and collagen Ⅳ in kidney were detected by immunohistochemistry,the mRNA expression of PAR\|2 was detected by reverse transcription polymerase chain reactionUsing an image analyzer with microscopy to analyse the correlationResultsTotal PAR\|2 mRNA levels started to rise in diabetic kidneys at 2W，reaching its peak at 8W， and reduced slightly at 12w and 20wPAR\|2 mainly expressed in renal tubular epithelial cells，especially in proximal tubular cellsIt also located in renal capillary ansa，interstitial infiltrate cells and fibroblastConclusionPAR\|2 mainly expressed in the renal proximal convoluted tubule epithelial cells in early diabetic rats,expression of PAR\|2 in the diabetic rats shows that PAR\|2 may play a role in tubulointerstitial fibrosis of diabetic nephropathy |
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\[5\]Roberts ISD,Brenchley PECMast cells:the forgotten cells of renal fibrosis\[J\]Clin Pathol,2000，53： 858\|862
\[6\]Kondo S,Kagami S,Kido H,et alRole of mast cell tryptase in renal interstitial fibrosis\[J\]Am Soc Nephrol，2001，12：1668\|1676 |
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《中国冶金工业医学杂志》 |