中国冶金工业医学杂志

抗氧化剂对高氧损伤新生大鼠脑细胞的保护作用研究

作者：孟卫霞1 钱燕2

关键词：高氧症脑损伤抗氧化剂

分类号：R965

出版年,卷(期):页码：2015,32(3):252-254

摘要：

目的探讨丹参提取物764\|3和超氧化物歧化酶（SOD）对高氧损伤新生大鼠脑细胞的保护作用。方法将SD新生大鼠持续暴露于氧浓度≥90%的高氧环境中建立高氧脑损伤模型，然后分别予764\|3、SOD进行预防(1～7 d)和治疗(8～14 d)干预后，计算各组大鼠存活率、体质量和脑湿/干重比值；采用TUNEL技术检测大鼠脑细胞凋亡情况并计算凋亡指数（AI）。结果与空气对照组比较，高氧对照组的AI显著上升（P＜001）；干预组的AI均显著低于高氧对照组（P＜001），预防组的AI显著高于治疗组（P＜001）。结论764\|3和SOD对于高氧脑损伤均有一定的保护作用，且治疗作用优于预防作用。

关键词：高氧症；脑损伤；抗氧化剂

ObjectiveTo investigate the effect of 764\|3 and SOD on hyperoxic brain injury in neonatal ratsMethodsHyperoxic brain injury models were established by exposing to ≥90% oxygen in the neonatal period of Spraque\|Dawley ratsAfter prevention (1~7 d) and treatment(8~14 d)with 764\|3 and SOD separately,in all groups survival rate of rat,body weight and wet /dry weight ratio were calculated；apoptosis of rat brain cell were tested by TUNEL technology and apoptotic index(AI)were calculatedResultsCompared with the control group,AI in the hyperoxia\|exposed group was significantly raised（P＜001）；AI in intervention group was significantly lowered than that in hyperoxia\|exposed group,in prevention group was significantly higher than that in treatment group（P＜001）ConclusionBoth 764\|3 and SOD have similarly protective effect on hyperoxia\|induced brain injury，therapeutic effect is superior to the preventive effectKey Words:Hyperoxia ；Brain diseases；Antioxidants

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参考文献：

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