Abstract：Objective To analyze the application value of different combination detection models of hepatitis C virus markers in transfusion patients, and to explore the reasonable detection procedure of hepatitis C virus markers in blood transfusion patients. Methods 1280 serum of patients before transfusion were selected and divided into two groups, total group（Group Ⅰ）and abnormal liver function group（Group Ⅱ）. 8 items of HCV-cAg and the liver function such as ALT, ADA, AST, GGT etc. of the serum before transfusion in Group Ⅰ（1280 cases）were measured; Group Ⅱ: 49 patients with abnormal liver function, negative for anti-HCV and excluded hepatitis B were selected from Group Ⅰ, the total amount of HCV-cAg and missed detection rate were retrospective analyzed. Results Group Ⅰ: the positive rate of anti-HCV combined HCV-cAg detection mode was 2.34%. The positive rate of HCV-cAg in negative anti-HCV was 0.63%.Group Ⅱ: compared with Group Ⅰ, the detection of HCV-Ag was reduced by 96.2%, and the missed detection rate of hepatitis C was 0.31%. Conclusion The screening of hepatitis C virus infection in patients with clinical transfusion can be preceded by the determination of liver function such as 8 items before transfusion and ADA,AST, etc. On the basis of these results, whether to do the determination of HCV-cAg or HCV-RNA were selected. This model not only can save a lot of medical expenses and manpower costs, but also can find hepatitis C patients of "window period", the missed rate is very low, and being worthy popularizing and applying.