Abstract：Objective To compare and analyze the effect of 3.5 mm self-closing sutureless transparent corneal incision and 2.0 mm clear corneal non-suture self-closing incision in cataract surgery. Methods A total of 96 cases of cataract patients who underwent phacoemulsification combined with intraocular lens implantation were selected from April 2018 to April 2019 were divided into group A and group B according to different surgical incisions, 48 cases in each group. The patients in group A were used 3.5 mm self-closing sutureless transparent corneal incision, and in group B were used 2.0 mm transparent corneal non-suturing self-closing incision. The visual acuity, surgical astigmatism, diopter level and postoperative complications in two groups were compared. Results The visual acuity of group B were higher than that of group A, the surgical astigmatism of group B were lower than that of group A, and the diopter of group B were higher than that of group A at 1, 4 and 12 weeks after operation, the differences of all data between the groups were statistically significant（t＝4.085, 3.194, 3.479, 4.601, 5.494, 3.891, 3.986, 5.411, 5.584, all P＜0.05）; and the diopter change rate at 1, 4 and 12 weeks after operation of group B was lower than that of group A, and the incidence of complications of group B was lower than that of group A, the differences between the groups were statistically significant（χ2＝4.800, 4.042, 4.631, 4.800, all P＜0.05）. Conclusion 2.0 mm clear corneal non-suturing self-closing incision is superior to 3.5 mm self-closing sutureless transparent corneal incision in cataract surgery. It is worthy of clinical application.