Abstract：Objective To explore the relationship between rotavirus and intestinal function and immune level in children with intussusceptio. Methods Children with intussusception admitted to the Fourth Hospital of Baotou from June 2020 to October 2021 were divided into rotavirus infection（RI）（n＝20）and non-rotavirus infection（NI）groups（n＝40）by fecal rotavirus examination. The levels of stool secretory immunoglobulin A（sIgA）before and after surgery were compared between the two groups, the immune levels in the two groups after treatment, including IgA, IgM, IgG, CD3+, CD4+, CD1q were compared. The liver and kidney function levels in the two groups after treatment, including alanine aminotransferase（ALT）, glutamic oxalacetic transaminase（AST）, and blood creatinine（Scr）were compared. The enlargement of mesenteric lymph nodes and the incidence of recurrence in the children of the two groups were observed. The levels of inflammatory indexes, including WBC, C-reactive protein（CRP）were measured before and on the 1st and 3rd postoperative days in both groups. Results The sIgA of the RI group was lower than that of the NI group before the enema, and the sIgA of the RI group was higher than that of the NI group after the enema, and the differences between the two groups were statistically significant（t＝6.219, 3.132, all P＜0.05）; compared within the groups, the differences between the sIgA of the RI group before and after the enema were statistically significant （t＝6.341, P＜0.05）, and the differences between the sIgA of the NI group before and after the enema, there was no statistically significant difference（t＝0.148, P＞0.05）. The differences between IgG and CD1q in the RI group and NI group after treatment were not statistically significant（t＝0.432, 0.634, all P＞0.05）; the differences between IgA, IgM, CD3+, CD4+ in the RI group and NI group after treatment were statistically significant（t＝3.081, 2.298, 5.250, 2.363, all P＜0.05）. ALT, AST and Scr were higher in the RI group than in the NI group after treatment, and the differences were statistically significant in both groups（t＝4.430, 2.765, 2.013, all P＜0.05）. The incidence of mesenteric lymph node enlargement and recapture were both higher in the RI group than in the NI group, and the differences were statistically significant in both groups （χ2＝13.538, 4.658, all P＜0.05）. WBC and CRP were higher in the RI group than in the NI group before surgery, and the differences between the two groups were statistically significant（t＝2.290, P＜0.05）; while WBC in the first and third postoperative days were lower than before surgery in both groups, and the differences between the two groups were statistically significant（F＝57.016, 9.749, all P＜0.05）. Conclusion Children with rotavirus infection with intussusception have elevated inflammatory indexes and higher incidence of mesenteric lymph node enlargement. sIgA is the main immunoglobulin of the intestinal mucosal barrier, and after rotavirus infection.sIgA secretion is reduced, which may induce the occurrence of intussusceptionafter rotavirus infection;children with intussusception have a high recapture rate, which affects immune function, liver and kidney function.Therefore, for rotavirus-positive children with intussusception, the monitoring of postoperative indicators should be strengthened.