Abstract：Objective To investigate the effects of hepatic arterial chemoembolization （TACE） combined with laparoscopic radiofrequency ablation （RFA） on immune function and the levels of fetal protein A （AFP） and carcinoembryonic antigen （CEA） in patients with advanced liver cancer. Methods A total of 80 patients with advanced liver cancer admitted in our hospital from January to December 2020 were selected and randomly divided into observation group and experimental group, with 40 patients in each group. The observation group was treated with TACE combined with RFA. The control group was treated with RFA alone. The changes of immune function, AFP and CEA levels after treatment were compared between the two groups. Results The complete tumor necrosis rate in the observation group was lower than that in the control group, and the tumor shrinkage rate was higher than that in the control group, and the difference between the two groups was statistically significant （χ2＝ 4.073, 4.053, both P＜0.05）; the AFP and CEA levels in both groups at 3 and 6 months after surgery were lower than those before surgery, and the decrease in the observation group was better than that in the control group, and the difference between the two groups was statistically significant （t＝10.923, 13.720, 1.022, 6.593, all P＜0.05）;  The levels of CD4+ and NK cells in the observation group increased than that in the control group, the increase in the observation group was greater than that in the control group; while the level of CD8+ decreased more than that in the control group, and the decrease in the observation group was greater than that in the control group, and the difference between the two groups was statistically significant （t＝6.298, 3.040, 2.117, all P＜0.05）. Conclusion The combination of TACE and RFA can effectively improve the immune function of patients, inhibit tumor spread, and make up for the shortcomings in each other's methods and operations.