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电子期刊 >> 正文 |
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摘要：
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目的:探讨TNF-α和Fas/FasL的表达及骨髓细胞凋亡与骨髓增生异常综合征(myelodysplastic syndrome,MDS)发生发展的关系.方法:采用ELISA法检测MDS患者骨髓单个核细胞培养上清液中TNF-α浓度,采用流式细胞术测定骨髓CD 34+细胞Fas、FasL的表达率和细胞凋亡率.结果:各型MDS患者骨髓单个核细胞培养上清液中TNF-α浓度和CD 34+细胞Fas、FasL的表达率均明显高于正常对照组(P＜0.01).其中RA/RAS组的TNF-α浓度和CD 34+细胞Fas+表达率均明显高于RAEB和RAEB-t组(P＜0.001),而RAEB与RAEB-t组组间无明显差异(P值分别为0.115和0.239);RAEB和RAEB-t组细胞的CD 34+FasL+表达率明显高于RA/RAS组(P值分别为0.001和0.010).RA/RAS和RAEB组的细胞凋亡率均高于对照组(P值均为0.000),而RAEB-t组的细胞凋亡率与对照组相近(P=0.216).各型MDS患者TNF-α浓度与CD34+Fas+表达率呈正相关(r=0.570,P=0.012).而CD 34+细胞凋亡率与TNF-α浓度或CD 34+Fas+表达率之间均无直线相关关系(P值分别为0.103和0.091).结论:MDS患者骨髓细胞的凋亡受多因素的调节,TNF-α与Fas/FasL系统介导的凋亡以及机体对其调控的失常参与了MDS骨髓无效造血的病理生理过程.
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参考文献：
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Morel P.Hebbar M.Lai JL Cytogenetic analysis has strong independent prognostic value in de novo myelodysplastic syndromes and be incorporated in a new scoring system/a repoet on 408 cases 1993(9) 张之南 血液病诊断及疗效标准 1998 Kitagawa M.Saito I.Kuwata T Overexpression of tumor necrosis factor(TNF)-alpha and interferon(IFN)-gamma by bone marrow cells from patients with myelodysplastic syndromes 1997(12) Claessens YE.Bouscary D.Dupont J M In vitro proliferation and differentiation of erythroid progenitors from patients with myelodysplastic syndromes:evidence for Fas-dependent apoptosis 2002(5) Gersuk GM.Beckham C.Loken M A role for tumor necrosis factor-α,Fas and Fas-ligand in marrow failure associated with myelodysplastic syndrome 1998(1) Bouscary D.Chen Y L.Guesnu M Activity of the caspase-3/CPP32 enzyme is increased in‘early stage ' myelodysplastic syndromes with excessive apoptosis,but caspase inhibition does not enhance colony formation in vitro 2000(7) Peddie CM.Wolf R.Melellan LI Oxidative DNA damage in CD34+ myelodysplastic cells is associated with intracellular redox changes and elevated plasma tumor necrosis factor-α concentration 1997 Griffith TS.Ferguson TA The role of FasL-induced apoptosis in immune privilege 1997(5) Kurotaki H.Tsushima Y.NagaiK apoptosis,bcl-2expression and P53 accumulation in myelodysplastic syndrome,myelodysplastic-syndrome-derived acute myelogenous leukemia and de novo acute myelogenous leukemia 2000(3) Paul S.Calmels B.Regulier E Tumor-induced immunosuppression 2002(2)
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